A fluorimetric assay for the spontaneous release of an N7-alkylguanine residue from duplex DNA.
نویسندگان
چکیده
A fluorimetric assay for monitoring depurination of the N7-alkylguanine adduct derived from the anticancer natural product leinamycin is described. This general approach could potentially provide the foundation for a high throughput assay that detects DNA-alkylating agents or a convenient continuous fluorimetric assay for base excision repair enzymes.
منابع مشابه
A New Cross-Link for an Old Cross-Linking Drug: The Nitrogen Mustard Anticancer Agent Mechlorethamine Generates Cross-Links Derived from Abasic Sites in Addition to the Expected Drug-Bridged Cross-Links.
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 15 8 شماره
صفحات -
تاریخ انتشار 2005